qPCR NEWS - August 2011 - focus on digital-PCR
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Dear researcher,
dear Gene Quantification page reader,
Our newsletter informs about the latest news in quantitative real-time PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene Quantification homepage. The focus of this newsletter issue is:
- Update on digital PCR - http://digital-PCR.gene-quantification.info
- Update of a lot new webinars & multi media presentations - http://WEBINAR.gene-quantification.info
- Update of a lot new qPCR talks - http://TALKS.gene-quantification.info
- MIQE qPCR APP for iPhone, iPad and iPod - new version 1.1 available since 3rd August 2011 - http://MIQE.gene-quantification.info
- US qPCR Symposium in Santa Clara, CA - 1st-4th November 2011 - http://EVENTS.gene-quantification.info
- Recorded qPCR 2011 Event => qPCR 2011 eConference - http://eConference.qPCR2011.net
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Digital PCR (dPCR) is a refinement of conventional PCR methods that can be used to directly quantify and clonally amplify nucleic acids (including DNA, cDNA, methylated DNA, or RNA). The key difference between dPCR and traditional PCR lies in the method of measuring nucleic acids amounts, with the former being a more precise method than PCR. PCR carries out one reaction per single sample. dPCR also carries out a single reaction within a sample, however the sample is separated into a large number of partitions and the reaction is carried out in each partition individually. This separation allows a more reliable collection and sensitive measurement of nucleic acid amounts. The method has been demonstrated as useful for studying variations in gene sequences - such as copy number variants, point mutations, and it is routinely used for clonal amplification of samples for "next-generation sequencing."
... ... more about digital here => http://digital-PCR.gene-quantification.info
Megapixel digital PCR.
Heyries KA, Tropini C, Vaninsberghe M, Doolin C, Petriv OI, Singhal A, Leung K, Hughesman CB, Hansen CL.
Nat Methods. 2011 8(8): 649-651
Evaluation of Digital PCR for Absolute DNA Quantification.
Sanders R, Huggett JF, Bushell CA, Cowen S, Scott DJ, Foy CA.
Anal Chem. 2011 Aug 1.
Probing individual environmental bacteria for viruses by using microfluidic digital PCR.
Tadmor AD, Ottesen EA, Leadbetter JR, Phillips R.
Science. 2011 333(6038): 58-62
Multiplex digital PCR: breaking the one target per color barrier of quantitative PCR.
Zhong Q, Bhattacharya S, Kotsopoulos S, Olson J, Taly V, Griffiths AD, Link DR, Larson JW.
Lab Chip. 2011 11(13): 2167-2174
Digital analysis of the expression levels of multiple colorectal cancer-related genes by multiplexed digital-PCR coupled with hydrogel bead-array.
Qi Z, Ma Y, Deng L, Wu H, Zhou G, Kajiyama T, Kambara H.
Analyst. 2011 136(11): 2252-2259
Genomic evidence of pre-invasive clonal expansion, dispersal and progression in bronchial dysplasia.
McCaughan F, Pipinikas CP, Janes SM, George PJ, Rabbitts PH, Dear PH.
J Pathol. 2011 224(2): 153-159
Quantitative and sensitive detection of rare mutations using droplet-based microfluidics.
Pekin D, Skhiri Y, Baret JC, Le Corre D, Mazutis L, Salem CB, Millot F, El Harrak A, Hutchison JB, Larson JW, Link DR, Laurent-Puig P, Griffiths AD, Taly V.
Lab Chip. 2011 11(13): 2156-2166
Noninvasive prenatal diagnosis of hemophilia by microfluidics digital PCR analysis of maternal plasma DNA.
Tsui NB, Kadir RA, Chan KC, Chi C, Mellars G, Tuddenham EG, Leung TY, Lau TK, Chiu RW, Lo YM.
Blood. 2011 117(13): 3684-3691
Statistical considerations for digital approaches to non-invasive fetal genotyping.
Chu T, Bunce K, Hogge WA, Peters DG.
Bioinformatics. 2010 26(22): 2863-2866
Prenatal diagnosis of fetal aneuploidies: post-genomic developments.
Hahn S, Jackson LG, Zimmermann BG.
Genome Med. 2010 2(8):50
... ... more about digital here => http://digital-PCR.gene-quantification.info
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We present new webinars, multi-media presentations, podcasts, and animations around:
- qPCR
- RNAi and microRNA
- qPCR workflow
- Next Generation Sequencing
- RNA quality control
- Online tutorials about the MIQE guidelines
- Introduction to Molecular and Cellular Biology
... ... and further molecular biology related topics on http://webinar.gene-quantification.info
We present new qPCR related talk and poster PDFs:
- from international conferences qPCR 2004 - qPCR 2011
- Fundamentals of Real-Time PCR
- Online tutorials about qPCR gene expression data analysis
- 2010 and 2011 eConferences via video stream
- a bundle of "old" presentations from 2004 - 2007
... ... and much more on http://talks.gene-quantification.info
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Did you miss the qPCR 2011 Symposium in Freising?
BioEPS is now presenting the qPCR 2011 eConference online via video stream!
With our new eConference streaming server a world-wide know-how transfer becomes possible in a fast and simple way!
qPCR 2011 Event now available NOW as eConfernce => http://eConference.qPCR2011.net
At the event around 500 attendee from 47 contries participated from 28th March - 1th April 2011. During the scientific symposium the presented talks were recorded in excellent sound quality and high movie resolution.
The qPCR 2011 eConference package contains 65 talks, grouped in following topics:
Single-Cell qPCR, Next Generation Sequencing, High throughput qPCR, MIQE guidelines, RNAi-miRNA, qPCR Data analysis, Circulating Nucleic Acids, Pre-analytical Steps, New qPCR Applications in Molecular Diagnostics, and more ... ...
qPCR 2011 Event now available NOW as eConfernce => http://eConference.qPCR2011.net
You can watch an in-depth demo presentation of our seminars and talks right here
=> http://eseminars.bioEPS.com/player/?action=demo
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MIQE_qPCR APP for iPhone, iPad and iPod - iOS Universal
new version 1.1 available since 3rd August 2011
http://MIQE-press.gene-quantification.info
Get help from a special team of experts in qPCR while on the move. MIQE - qPCR helps you in reviewing scientific works and checking your own experiments, when qPCR is involved. Check your project's compliance to MIQE in minutes, have all required references to hand, and follow qPCR events and news.
Over 1,500 downloads in three months!
http://itunes.apple.com/app/miqe-qpcr/id423650002?mt=8
What's New in MIQE_qPCR Version 1.1
- Now MIQE qPCR app is even more interactive
- Checklists are specific for each project type- RNA or DNA
- Moreover, some items may not apply to your specific experiments. You can now remove them and have the most accurate MIQE compliancy
- References have been updated, so you can keep in touch with latest MIQE related literature, symposium updates and more
- Export is available. You can archive the state of your project whenever you want, share it with your colleagues, and store it
- Suggestions collected from experts at the qPCR 2011 symposium in Freising were included
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Forward Please send the qPCR NEWS to further scientists and friends who are interested in qPCR !
Best regards,
Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages
http://www.gene-quantification.info
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