Also, you may want to check out the Skene's gland, which excretes AP
during female ejaculation.
http://www.amygilliland.com/documents/GillilandFE.pdf
<http://www.amygilliland.com/documents/GillilandFE.pdf>
http://www.mdlinx.com/obstetrics-gynecology/news-article.cfm/3009758/vag\
inal-orgasm
<http://www.mdlinx.com/obstetrics-gynecology/news-article.cfm/3009758/va\
ginal-orgasm>
http://onlinelibrary.wiley.com/doi/10.1111/j.1743-6109.2009.01650.x/full
<http://onlinelibrary.wiley.com/doi/10.1111/j.1743-6109.2009.01650.x/ful\
l>
--- In forensic-science@yahoogroups.com, "John Lloyd Scharf"
<johnlloydscharf@...> wrote:
>
> I do not understand what you are saying. You are switching up terms,
> although I have not seen documentation of false positives. Obviously,
> you can have seminal fluid without spermatozoa. You can get Acid
> Phosphatase (AP) positive with a female from the glands of Bartholin,
> which are analogous to the Cowper's gland in the male, where AP is
> excreted. The AP indicates sexual activity, but the gender of those
> involved is not certain.
> --- In forensic-science@yahoogroups.com, Donna Hansen dhansen@
> wrote:
> >
> > Would you be able to share the information or location about the
false
> positives with the RSID- semen test.
> >
> > From: forensic-science@yahoogroups.com
> [mailto:forensic-science@yahoogroups.com] On Behalf Of johnsonethan95
> > Sent: Friday, September 30, 2011 1:25 AM
> > To: forensic-science@yahoogroups.com
> > Subject: [forensic-science] Re: Wording of Serology Reports
> >
> >
> >
> > RSID test is no better. There is a fair amount of information on
false
> positives from RSID test. Seems like we have run out of options.
> >
> > --- In
>
forensic-science@yahoogroups.com<mailto:forensic-science%40yahoogroups.c\
\
> om>, Donna Hansen dhansen@ wrote:
> > >
> > > I just wanted to say we were having the same issue with the semen
> cassettes but we were getting false positives with our Seratec p30
test
> cassettes and were going to switch to the ABAcard. We ended up
switching
> to a semen specific test called RSID-Semen; it tests for the presence
of
> Semenogelin which is only found in semen (as its documentation states
> now just like p30 was specific back in the day). As for your
conclusion
> - we basically conclude the same way except we say "Semen was or was
not
> detected on Item ....". Where Semen was positive but no spermatozoa
were
> detected - we included that information on our report "Semen was
> detected but no spermatozoa were observed". I cannot really address
> option 4 but we were close (before we started using RSID-Semen) to
using
> an inconclusive result.
> > >
> > > Do you do a preliminary color test i.e. acid phosphatase - if you
do
> are those results taking into consideration as to how you conclude
your
> semen result?
> > >
> > > From:
>
forensic-science@yahoogroups.com<mailto:forensic-science%40yahoogroups.c\
\
> om>
>
[mailto:forensic-science@yahoogroups.com<mailto:forensic-science%40yahoo\
\
> groups.com>] On Behalf Of labgirl28
> > > Sent: Wednesday, September 28, 2011 10:57 PM
> > > To:
>
forensic-science@yahoogroups.com<mailto:forensic-science%40yahoogroups.c\
\
> om>
> > > Subject: [forensic-science] Wording of Serology Reports
> > >
> > >
> > >
> > > My laboratory is on the cusp of revamping our Serology SOP and the
> way that we word some of our serology testing on our final reports. I
> know that the "word of the day" when it comes to ISO, which is where
> most of us are headed, is TRANSPARENCY. Nothing would make me happier
> and more comfortable as a forensic scientist to be able to go to
court,
> clearly state what tests were used, and openly show/explain their
> limitations. Anyone that has read about the North Carolina debaucle
> understands that a scientist's report, despite their best intentions
AND
> following proper protocol, can be grossly misinterpreted without them
> personally being present to explain it and in turn, ruin their career
in
> forensics.
> > > Anyhow, several of my coworkers are facing some resistance by
> administration when it comes to how we report out semen testing and
what
> "weight" we give to these tests in regard to probative value. Let me
> also say that we were getting false (+)'s with ABAcard psa on known
> semen-free samples. Their was some discussion about temperature, pH,
and
> viscosity issues that could cause these results. Due to these issues,
we
> switched to Seratec's product. I will also state that our DNA section
> does tell the end of the story many times by stating whether foreign
DNA
> is present in our swabbings and cuttings which is a small comfort to
us
> serologists, but that sometimes, the mere reporting of semen being
> present is all it takes for a jury to convict, even if DNA is not
> obtained. I am trying to poll other forensic laboratories to see how
you
> guys report out the following testing so that I can attend our next
> brainstorming meeting with some possible suggestions:
> > > 1) Spermatozoa identified
> > > 2) No Spermatozoa identified, (+) p30 result
> > > 3) No Spermatozoa identified, (-) p30 result
> > > 4) No Spermatozoa identified, p30 result(test line intensity is
> lighter than internal standard of 4 ng/mL)
> > >
> > > For 1), we currently write "Semen was identified on.....".
> > > For 2), we currently write "Semen was identified on.....".
> > > For 3), we currently write "No semen was found on....".
> > > For 4), we currently write "Tests for the presence of semen were
> inconclusive.".
> > >
> > > Sadly, our current protocol dictates that if our test line is (+)
or
> less intense than the internal standard, we must repeat the test with
> another p30 card of the same lot # (I don't see this as sound
scientific
> practice). If the second test is also (+), we follow 1) wording as
> above. If the second test is (-), we are told to write "No semen was
> found on..." (I don't agree with this.) I know that Seratec is very
> sensitive. The manufacturer clearly shows examples of fainter lines
> being still interpreted as (+) for p30. I also know that the test
line,
> results being based on a bell curve of concentration, may be fainter
> because there's low quantities of p30 OR very high quantities,
> approaching the high-dose hook effect level which would give you a
false
> (-).
> > > Do any other laboratories interpret these faint lines as anything
> other than (+)? Does your lab call this (+) for p30, a component of
> semen OR (+) for semen?
> > >
> > > Do your reports give disclaimers about p30 being found in low
levels
> of other body fluids?
> > >
> > > Lastly, does your laboratory consider p30 testing to be
> sensitive/specific enough to be called a confirmatory test for semen?
> Ours has for years and doesn't want to even consider backing off on
> report wording to view it as presumptive, which many of us feel is
> imperative. There was some talk of describing it as "indicitive," but
> that is what the poor soul in North Carolina used and we all know how
> that turned out for him! We're all of the "worst-case scenario"
mindset
> and fear one day, major consequences could befall our laboratory or us
> analysts though we are following SOP as set forth and attempting to
> remain subordinate to our superiors.
> > > I know I've rambled for a lengthy spell here, but we've got a
burden
> on our shoulders that needs resolution. Misinterpretation of data is
not
> an acceptable answer for me. I don't see anywhere on Seratec's website
> where they consider any type of line in the Test area to be anything
but
> (+). That is the bottom line. My signature on a laboratory report
means
> something to me and I don't want it to lose its value.
> > >
> > >
> > >
> > > [Non-text portions of this message have been removed]
> > >
> >
> >
> >
> > [Non-text portions of this message have been removed]
> >
>
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